Tag

Part:BBa_K3102015:Design

Designed by: Sarah DUQUESNE   Group: iGEM19_Ionis_Paris   (2019-10-03)


hlyA (Hemolysin A)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 112
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 112
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 112
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 112
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

none


Source

This sequence comes from E.coli and was found through iGEM Registry: https://parts.igem.org/Part:BBa_K554002

References

Nicaud J. M., Mackman N., Gray L., Holland I. B. The C-terminal, 23kDa peptide of Escherichia coli hemolysin 2001 contains all the information necessary for its secretion by the hemolysin (Hly) export machinery. FEBS Lett. 204:331–335. (1986)

Stanley P., Koronakis V., Hughes C. Mutational analysis supports a role for multiple structural features in the C-terminal secretion signal of Escherichia coli hemolysin. Mol. Microbiol. 5:2391–2403 (1991)